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a549 h2b mcherry  (Thermo Fisher)


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    Thermo Fisher a549 h2b mcherry
    MOI-dependent temporal expression dynamics of the NS1 protein (PR8-GFP virus) in human respiratory epithelial cells <t>(A549</t> <t>H2B-mCherry)</t> using time-lapse microscopy. Green indicates NS1 expression (GFP). The cell nuclei (H2B-mcherry) are depicted in red. (A) Time course from 3 to 12 hpi. One representative field is shown for each MOI. (B) Quantification of the number of GFP-positive cells for the four MOIs used (approximately 25,000 cells/well). For MOI of 0.2, 0.6, and 2, averages of two replicate wells ± SDs are shown. For infection at an MOI of 10 and mock infection, data from one single well are shown. (C) Heat map showing the median of fluorescence intensity of the infected cells (GFP+ cells). (D) Table showing the measured percentages of infected cells at 12 hpi, as well as the theoretical percentages of cells infected either with ≥1 virion (all infected cells) or >1 virion (multiple infections).
    A549 H2b Mcherry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a549+h2b+mcherry/Trypsin/pmc06798124-286-0-15
    Average 99 stars, based on 1 article reviews
    a549 h2b mcherry - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Innate Immune Response to Influenza Virus at Single-Cell Resolution in Human Epithelial Cells Revealed Paracrine Induction of Interferon Lambda 1"

    Article Title: Innate Immune Response to Influenza Virus at Single-Cell Resolution in Human Epithelial Cells Revealed Paracrine Induction of Interferon Lambda 1

    Journal: Journal of Virology

    doi: 10.1128/JVI.00559-19

    MOI-dependent temporal expression dynamics of the NS1 protein (PR8-GFP virus) in human respiratory epithelial cells (A549 H2B-mCherry) using time-lapse microscopy. Green indicates NS1 expression (GFP). The cell nuclei (H2B-mcherry) are depicted in red. (A) Time course from 3 to 12 hpi. One representative field is shown for each MOI. (B) Quantification of the number of GFP-positive cells for the four MOIs used (approximately 25,000 cells/well). For MOI of 0.2, 0.6, and 2, averages of two replicate wells ± SDs are shown. For infection at an MOI of 10 and mock infection, data from one single well are shown. (C) Heat map showing the median of fluorescence intensity of the infected cells (GFP+ cells). (D) Table showing the measured percentages of infected cells at 12 hpi, as well as the theoretical percentages of cells infected either with ≥1 virion (all infected cells) or >1 virion (multiple infections).
    Figure Legend Snippet: MOI-dependent temporal expression dynamics of the NS1 protein (PR8-GFP virus) in human respiratory epithelial cells (A549 H2B-mCherry) using time-lapse microscopy. Green indicates NS1 expression (GFP). The cell nuclei (H2B-mcherry) are depicted in red. (A) Time course from 3 to 12 hpi. One representative field is shown for each MOI. (B) Quantification of the number of GFP-positive cells for the four MOIs used (approximately 25,000 cells/well). For MOI of 0.2, 0.6, and 2, averages of two replicate wells ± SDs are shown. For infection at an MOI of 10 and mock infection, data from one single well are shown. (C) Heat map showing the median of fluorescence intensity of the infected cells (GFP+ cells). (D) Table showing the measured percentages of infected cells at 12 hpi, as well as the theoretical percentages of cells infected either with ≥1 virion (all infected cells) or >1 virion (multiple infections).

    Techniques Used: Expressing, Virus, Time-lapse Microscopy, Infection, Fluorescence

    Stimulation of A549 cells with TNF-α, IFN beta, IFNL1 (100 U/ml), or combinations of those cytokines or infected with PR8-GFP (MOI, 2) for 8 h. Induction of IFNL1 was not detected upon stimulation with those cytokines by qRT-PCR. Gene expression of the indicated genes (IFNs, with Mx1 and CCL5 to confirm the effectiveness of the treatments) was analyzed. Averages of biological triplicates ± SDs are shown.
    Figure Legend Snippet: Stimulation of A549 cells with TNF-α, IFN beta, IFNL1 (100 U/ml), or combinations of those cytokines or infected with PR8-GFP (MOI, 2) for 8 h. Induction of IFNL1 was not detected upon stimulation with those cytokines by qRT-PCR. Gene expression of the indicated genes (IFNs, with Mx1 and CCL5 to confirm the effectiveness of the treatments) was analyzed. Averages of biological triplicates ± SDs are shown.

    Techniques Used: Infection, Quantitative RT-PCR, Gene Expression

    Related Articles

    Cell Culture:

    Article Title: Innate Immune Response to Influenza Virus at Single-Cell Resolution in Human Epithelial Cells Revealed Paracrine Induction of Interferon Lambda 1
    Article Snippet: The graph and heat map in were generated using R v3.3.1 and Rstudio. .. A549 H2B-mCherry-infected or mock-infected cells were detached from the cell culture plates using trypsin-0.05% EDTA (Gibco), washed with cell culture media, and passed through a 40-μm cell strainer to prepare single-cell suspensions, and the final concentration was set at 1,000 cells/μl. ..

    Single Cell:

    Article Title: Innate Immune Response to Influenza Virus at Single-Cell Resolution in Human Epithelial Cells Revealed Paracrine Induction of Interferon Lambda 1
    Article Snippet: The graph and heat map in were generated using R v3.3.1 and Rstudio. .. A549 H2B-mCherry-infected or mock-infected cells were detached from the cell culture plates using trypsin-0.05% EDTA (Gibco), washed with cell culture media, and passed through a 40-μm cell strainer to prepare single-cell suspensions, and the final concentration was set at 1,000 cells/μl. ..

    Concentration Assay:

    Article Title: Innate Immune Response to Influenza Virus at Single-Cell Resolution in Human Epithelial Cells Revealed Paracrine Induction of Interferon Lambda 1
    Article Snippet: The graph and heat map in were generated using R v3.3.1 and Rstudio. .. A549 H2B-mCherry-infected or mock-infected cells were detached from the cell culture plates using trypsin-0.05% EDTA (Gibco), washed with cell culture media, and passed through a 40-μm cell strainer to prepare single-cell suspensions, and the final concentration was set at 1,000 cells/μl. ..



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    MOI-dependent temporal expression dynamics of the NS1 protein (PR8-GFP virus) in human respiratory epithelial cells <t>(A549</t> <t>H2B-mCherry)</t> using time-lapse microscopy. Green indicates NS1 expression (GFP). The cell nuclei (H2B-mcherry) are depicted in red. (A) Time course from 3 to 12 hpi. One representative field is shown for each MOI. (B) Quantification of the number of GFP-positive cells for the four MOIs used (approximately 25,000 cells/well). For MOI of 0.2, 0.6, and 2, averages of two replicate wells ± SDs are shown. For infection at an MOI of 10 and mock infection, data from one single well are shown. (C) Heat map showing the median of fluorescence intensity of the infected cells (GFP+ cells). (D) Table showing the measured percentages of infected cells at 12 hpi, as well as the theoretical percentages of cells infected either with ≥1 virion (all infected cells) or >1 virion (multiple infections).
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    MOI-dependent temporal expression dynamics of the NS1 protein (PR8-GFP virus) in human respiratory epithelial cells <t>(A549</t> <t>H2B-mCherry)</t> using time-lapse microscopy. Green indicates NS1 expression (GFP). The cell nuclei (H2B-mcherry) are depicted in red. (A) Time course from 3 to 12 hpi. One representative field is shown for each MOI. (B) Quantification of the number of GFP-positive cells for the four MOIs used (approximately 25,000 cells/well). For MOI of 0.2, 0.6, and 2, averages of two replicate wells ± SDs are shown. For infection at an MOI of 10 and mock infection, data from one single well are shown. (C) Heat map showing the median of fluorescence intensity of the infected cells (GFP+ cells). (D) Table showing the measured percentages of infected cells at 12 hpi, as well as the theoretical percentages of cells infected either with ≥1 virion (all infected cells) or >1 virion (multiple infections).
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    MOI-dependent temporal expression dynamics of the NS1 protein (PR8-GFP virus) in human respiratory epithelial cells <t>(A549</t> <t>H2B-mCherry)</t> using time-lapse microscopy. Green indicates NS1 expression (GFP). The cell nuclei (H2B-mcherry) are depicted in red. (A) Time course from 3 to 12 hpi. One representative field is shown for each MOI. (B) Quantification of the number of GFP-positive cells for the four MOIs used (approximately 25,000 cells/well). For MOI of 0.2, 0.6, and 2, averages of two replicate wells ± SDs are shown. For infection at an MOI of 10 and mock infection, data from one single well are shown. (C) Heat map showing the median of fluorescence intensity of the infected cells (GFP+ cells). (D) Table showing the measured percentages of infected cells at 12 hpi, as well as the theoretical percentages of cells infected either with ≥1 virion (all infected cells) or >1 virion (multiple infections).
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    Image Search Results


    MOI-dependent temporal expression dynamics of the NS1 protein (PR8-GFP virus) in human respiratory epithelial cells (A549 H2B-mCherry) using time-lapse microscopy. Green indicates NS1 expression (GFP). The cell nuclei (H2B-mcherry) are depicted in red. (A) Time course from 3 to 12 hpi. One representative field is shown for each MOI. (B) Quantification of the number of GFP-positive cells for the four MOIs used (approximately 25,000 cells/well). For MOI of 0.2, 0.6, and 2, averages of two replicate wells ± SDs are shown. For infection at an MOI of 10 and mock infection, data from one single well are shown. (C) Heat map showing the median of fluorescence intensity of the infected cells (GFP+ cells). (D) Table showing the measured percentages of infected cells at 12 hpi, as well as the theoretical percentages of cells infected either with ≥1 virion (all infected cells) or >1 virion (multiple infections).

    Journal: Journal of Virology

    Article Title: Innate Immune Response to Influenza Virus at Single-Cell Resolution in Human Epithelial Cells Revealed Paracrine Induction of Interferon Lambda 1

    doi: 10.1128/JVI.00559-19

    Figure Lengend Snippet: MOI-dependent temporal expression dynamics of the NS1 protein (PR8-GFP virus) in human respiratory epithelial cells (A549 H2B-mCherry) using time-lapse microscopy. Green indicates NS1 expression (GFP). The cell nuclei (H2B-mcherry) are depicted in red. (A) Time course from 3 to 12 hpi. One representative field is shown for each MOI. (B) Quantification of the number of GFP-positive cells for the four MOIs used (approximately 25,000 cells/well). For MOI of 0.2, 0.6, and 2, averages of two replicate wells ± SDs are shown. For infection at an MOI of 10 and mock infection, data from one single well are shown. (C) Heat map showing the median of fluorescence intensity of the infected cells (GFP+ cells). (D) Table showing the measured percentages of infected cells at 12 hpi, as well as the theoretical percentages of cells infected either with ≥1 virion (all infected cells) or >1 virion (multiple infections).

    Article Snippet: A549 H2B-mCherry-infected or mock-infected cells were detached from the cell culture plates using trypsin-0.05% EDTA (Gibco), washed with cell culture media, and passed through a 40-μm cell strainer to prepare single-cell suspensions, and the final concentration was set at 1,000 cells/μl.

    Techniques: Expressing, Virus, Time-lapse Microscopy, Infection, Fluorescence

    Stimulation of A549 cells with TNF-α, IFN beta, IFNL1 (100 U/ml), or combinations of those cytokines or infected with PR8-GFP (MOI, 2) for 8 h. Induction of IFNL1 was not detected upon stimulation with those cytokines by qRT-PCR. Gene expression of the indicated genes (IFNs, with Mx1 and CCL5 to confirm the effectiveness of the treatments) was analyzed. Averages of biological triplicates ± SDs are shown.

    Journal: Journal of Virology

    Article Title: Innate Immune Response to Influenza Virus at Single-Cell Resolution in Human Epithelial Cells Revealed Paracrine Induction of Interferon Lambda 1

    doi: 10.1128/JVI.00559-19

    Figure Lengend Snippet: Stimulation of A549 cells with TNF-α, IFN beta, IFNL1 (100 U/ml), or combinations of those cytokines or infected with PR8-GFP (MOI, 2) for 8 h. Induction of IFNL1 was not detected upon stimulation with those cytokines by qRT-PCR. Gene expression of the indicated genes (IFNs, with Mx1 and CCL5 to confirm the effectiveness of the treatments) was analyzed. Averages of biological triplicates ± SDs are shown.

    Article Snippet: A549 H2B-mCherry-infected or mock-infected cells were detached from the cell culture plates using trypsin-0.05% EDTA (Gibco), washed with cell culture media, and passed through a 40-μm cell strainer to prepare single-cell suspensions, and the final concentration was set at 1,000 cells/μl.

    Techniques: Infection, Quantitative RT-PCR, Gene Expression